[Analysis of estrogen receptors in normal bone and bone tumor tissues]

Thirteen human normal bone and fifteen bone tumor tissues were assayed for estrogen receptor (ER) by Dextran-coated Charcoal method (Scatchard plot). The results showed that the concentrations of ER in normal bone tissues (15.12 +/- 14.68 fmol/mg pro) were higher than that of bone malignant tumors (8.04 +/- 6.71 fmol/mg pro) P < 0.05). The binding dissociation constants (Kd) of ER in bone tumors (18.46 +/- 27.10 x 10(-11) mol/L) was lower than those in normal ones (39.91 +/- 20.13 x 10(-11) mol/L) (P < 0.025). The percentages for positive score of ER in normal and tumor ones (23.1%, 26.7%) were not significantly different (P > 0.05). Our study indicated that the variations of concentration and affinity of ER in bone related to the tumor incidence. That cytosol ER content was decreased in malignant bone tumors whereas that of affinity increase suggests an impairment or change of an intact receptor mechanism in this tissues.