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Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene
1Howard Hughes Medical Institute, Department of Developmental Biology, Beckman Center, Stanford University, California 94305.
Genomics
|September 1, 1993
Abstract:
In mouse mammary tumors, the Wnt-3 gene can be activated by proviral insertion. Here we report on the isolation of a human homolog, WNT3. A genomic clone was isolated by use of mouse Wnt-3 sequences as a probe, after which cDNA containing most of the protein-encoding domain of the human gene was obtained by PCR. Comparison between the deduced mouse and human WNT-3 protein sequences showed four changes in 333 amino acids. WNT3 is located on chromosome 17q21. The gene was not found to be amplified or rearranged in a collection of human breast tumors.