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Related Experiment Videos

Thrombin generates monocyte chemotactic activity from complement factor H

H Ohtsuka1, T Imamura, M Matsushita

  • 1Department of Neuroscience and Immunology, Kumamoto University Graduate School of Medical Sciences, Japan.

Immunology
|September 1, 1993
PubMed
Summary
This summary is machine-generated.

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Complement factor H (H) transforms into a potent monocyte chemoattractant after thrombin cleavage. This process, crucial in delayed-type hypersensitivity reactions, enhances monocyte migration to inflammatory sites.

Area of Science:

  • Immunology
  • Biochemistry
  • Molecular Biology

Background:

  • Complement factor H (H) is a precursor to macrophage chemotactic factors at inflammatory sites.
  • The conversion of H to a chemotactic factor involves an unknown trypsin-like protease.
  • Thrombin and plasmin are proteases present at delayed-type hypersensitivity (DTH) reaction sites.

Purpose of the Study:

  • To investigate if thrombin or plasmin convert complement factor H (H) into a monocyte chemoattractant.
  • To understand the mechanism by which H gains monocyte chemotactic activity.

Main Methods:

  • Incubation of complement factor H (H) with thrombin and plasmin.
  • Assay of monocyte migration in response to treated H.
  • Characterization of the generated chemotactic activity (chemotactic vs. chemokinetic).

Related Experiment Videos

  • Analysis of H's inhibitory activity against C3b-Bb after protease treatment.

    • Main Results:

    • Thrombin, but not plasmin, converted H into a molecule with monocyte chemotactic activity.
    • The generation of activity was time-dependent on thrombin incubation and required thrombin's protease activity.
    • Thrombin treatment increased the hydrophobicity of H, correlating with its chemotactic function.

    Conclusions:

    • Complement factor H (H) is converted to a monocyte chemoattractant by thrombin cleavage.
    • Thrombin-induced cleavage of H generates a more hydrophobic molecule responsible for monocyte migration.
    • Thrombin-treated H likely plays a role in inducing monocyte migration within DTH reaction sites.