Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cryopreservation of human bronchi

E Müller-Schweinitzer1, J Hasse, L Swoboda

  • 1Preclinical Research, Sandoz Pharma Ltd., Basel, Switzerland.

The Journal of Asthma : Official Journal of the Association for the Care of Asthma
|January 1, 1993
PubMed
Summary

Cryopreservation of human bronchi at -196°C using Krebs-Henseleit solution best preserves contractile function. While some relaxation responses were reduced, key mechanisms remain intact for future pharmacological studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Prevention, diagnosis, therapy, and follow-up of lung cancer. Interdisciplinary guideline of the German Respiratory Society and the German Cancer Society--abridged version].

Pneumologie (Stuttgart, Germany)·2011
Same author

Prevention, diagnosis, therapy, and follow-up of lung cancer: interdisciplinary guideline of the German Respiratory Society and the German Cancer Society.

Pneumologie (Stuttgart, Germany)·2010
Same author

[Prevention, diagnosis, therapy, and follow-up of lung cancer].

Pneumologie (Stuttgart, Germany)·2010
Same author

[Extrathoracal pulmonary sequestration in the differential diagnosis of paravertebral tumors in the neonate].

Klinische Padiatrie·2006
Same author

[Resection of combined or sequential lung and liver metastases of colorectal cancer: indication for everyone?].

Zentralblatt fur Chirurgie·2005
Same author

Protecting the endothelial integrity of internal thoracic arteries.

The Thoracic and cardiovascular surgeon·2005

Area of Science:

  • Pulmonology
  • Cryobiology
  • Pharmacology

Background:

  • Human bronchi smooth muscle is crucial for respiratory function.
  • Effective cryopreservation methods are needed for long-term storage and research.
  • Understanding airway smooth muscle mechanics is vital for treating respiratory diseases.

Purpose of the Study:

  • To evaluate the efficacy of different cryopreservation media for human bronchi.
  • To assess the impact of cryopreservation on airway smooth muscle contractile and relaxant responses.
  • To determine the optimal conditions for preserving human bronchi for pharmacological experiments.

Main Methods:

  • Human bronchi were cryopreserved at -196°C in media with dimethyl sulfoxide and sucrose using fetal calf serum (FCS) or Krebs-Henseleit solution (KH) as vehicles.
  • Post-thaw contractile responses to carbachol and histamine were measured.
  • Responses to relaxant agonists (isoprenaline, papaverine, bimakalim) and passive tension were assessed.

Main Results:

  • Optimal preservation of contractile responsiveness was achieved when using KH solution as the vehicle.
  • Maximal relaxant responses were attenuated by up to 50% in cryopreserved tissues.
  • Passive resting tension was higher in cryopreserved bronchi compared to fresh tissues.

Conclusions:

  • Cryopreservation of human bronchi at -196°C, particularly in KH solution, preserves essential contractile and relaxant mechanisms.
  • Despite some loss of elasticity, cryopreserved bronchi are suitable for subsequent pharmacological investigations.
  • This method offers potential for storing human airway smooth muscle for research purposes.

Related Experiment Videos