Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bovine alpha-fetoprotein. Isolation and characterization

S Aliau, J Marti, J Moretti

    Biochimie
    |September 29, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Traumatic focal myositis of the temporalis muscle due to presumptive impingement between the zygomatic arch and coronoid process in two cocker spaniels with restricted mouth opening.

    The Journal of small animal practice·2026
    Same author

    Pragmatic management of rabies risk in operation DAMAN 50 (Lebanon, October 2024-February 2025): a case study on evidence-based military field medicine.

    Infectious diseases now·2025
    Same author

    Analysis of intra-fractional positioning correction performed by cone beam computed tomography in SBRT treatments.

    Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2024
    Same author

    Adolescent and Parent Preferences for Hypodontia: Discrete Choice Experiment.

    Journal of dental research·2022
    Same author

    Low-dose radiation therapy for hand osteoarthritis: shaking hands again?

    Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2021
    Same author

    Moderate hypofractionated post-prostatectomy radiation therapy is feasible and well tolerated: experience from a single tertiary cancer centre.

    Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2021
    Same journal

    Differential cardiac microRNA expression in anoxic Trachemys scripta elegans turtles.

    Biochimie·2026
    Same journal

    Renal failure-driven luminal ammonia production impairs gut barrier function in CKD.

    Biochimie·2026
    Same journal

    Conditional Knockout of Indoleamine 2, 3-Dioxygenase-1 in Osteoprogenitor Cells in Mice Results in Sex-dependent Differences in Bone Mass.

    Biochimie·2026
    Same journal

    Sedentariness disrupts, while exercise restores, thermogenic and metabolic plasticity in inguinal adipose tissue of mice.

    Biochimie·2026
    Same journal

    Chrononutrition as a modulator of retinal metabolic resilience: A translational framework linking circadian biology to ocular disease.

    Biochimie·2026
    Same journal

    Heterologous expression, purification, and biophysical characterisation of the cobalt-dependent nitrile hydratase from Rhodococcus rhodochrous ATCC BAA-870.

    Biochimie·2026
    See all related articles

    This study details the purification and characterization of bovine alpha-fetoprotein (AFP), a glycoprotein. Researchers determined its molecular weight, diffusion coefficient, and frictional ratio, confirming its homogeneity and microheterogeneity.

    Area of Science:

    • Biochemistry
    • Proteomics
    • Glycobiology

    Background:

    • Alpha-fetoprotein (AFP) is a major fetal serum protein with potential roles in development and disease.
    • Understanding the biophysical and chemical properties of bovine AFP is crucial for comparative studies and potential applications.

    Purpose of the Study:

    • To purify and extensively characterize bovine alpha-fetoprotein (AFP).
    • To determine the molecular weight, homogeneity, and microheterogeneity of purified bovine AFP.
    • To analyze the chemical composition, including carbohydrate content, of bovine AFP.

    Main Methods:

    • Purification using ion exchange chromatography (C.M. cellulose, DEAE Sephadex A-50), gel filtration, and immunosorbent techniques.
    • Homogeneity assessment via gel electrophoresis (native and denaturing), ultracentrifugation, and immunological methods.

    Related Experiment Videos

  • Molecular weight determination using sedimentation equilibrium, sedimentation velocity, SDS-PAGE, and gel filtration.

    • Main Results:

    • Bovine AFP was purified to homogeneity, confirmed by multiple techniques.
    • Sedimentation equilibrium yielded a molecular weight of 66,500 Da; SDS-PAGE suggested 67,000 Da.
    • Isoelectric focusing revealed microheterogeneity, with the major component's isoelectric point at 4.6.
    • AFP was identified as a glycoprotein containing 7% carbohydrates, including hexoses, N-acetyl glucosamine, and N-acetyl neuraminic acid.

    Conclusions:

    • Bovine AFP is a homogeneous glycoprotein with a defined molecular weight and sedimentation properties.
    • The study confirms the microheterogeneity of bovine AFP and provides detailed compositional analysis.
    • These findings establish a comprehensive biophysical and chemical profile of bovine AFP.