Related Experiment Videos
Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains
T V Guaycurus1, A C Vicente, S G de Simone
1Departamento de Bacteriologia, Instituto Oswaldo Cruz, Rio de Janeior, Brasil.
Memorias Do Instituto Oswaldo Cruz
|January 1, 1993
Abstract:
Extracellular proteins produced by Bacillus cereus AL-42 and AL-15 were fractioned by chromatography on QAE-Sephadex and Sephadex G75. This last chromatographic process resulted in three peaks. The major peak showed vascular permeability activity to rabbits, lethality to mice, and cytotoxicity to Vero and Hela cells. The analysis by SDS-PAGE after ultrafiltration confirm recent findings that the enterotoxin is a compound with molecular mass > 30,000.