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Production and properties of a factor X-cellulose-binding domain fusion protein
Z Assouline1, H Shen, D G Kilburn
1Department of Microbiology, University of British Columbia, Vancouver, Canada.
Protein Engineering
|September 1, 1993
Abstract:
A fusion protein, FX-CBDCex, which comprises factor X with a cellulose-binding domain (CBDCex) fused to its C-terminus, was produced in BHK cells. It was purified from the culture medium by affinity chromatography on cellulose. FX-CBDCex could be activated to FXa-CBDCex with Russell viper venom. FXa-CBDCex was as active as FXa against a chromogenic substrate and against proteins containing the Ile-Glu-Gly-Arg sequence hydrolysed by FXa. FXa-CBDCex retained its activity when adsorbed to cellulose.