Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An examination of the generation-time effect on molecular evolution

T Ohta1

  • 1National Institute of Genetics, Mishima, Japan.

Proceedings of the National Academy of Sciences of the United States of America
|November 15, 1993
PubMed
Summary

The study found that the generation-time effect, impacting DNA changes, is more pronounced for synonymous substitutions than nonsynonymous ones in mammals. This suggests a higher rate of amino acid evolution in primates compared to rodents, supporting neutral evolution theories.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of the gene encoding human pituitary-specific transcription factor, Pit-1.

Gene·1992
Same author

Hyperphosphorylation of cytokeratins 8 and 18 by microcystin-LR, a new liver tumor promoter, in primary cultured rat hepatocytes.

Carcinogenesis·1992
Same author

Isolation of 2 novel RFLP markers and their localization at 2q35 by microdissection and subsequent enzymatic amplification.

The Japanese journal of human genetics·1992
Same author

Sensitive period for the induction of endoreduplication by rotenone in cultured Chinese hamster cells.

Chromosoma·1992
Same author

Russuphelin A, a new cytotoxic substance from the mushroom Russula subnigricans Hongo.

Chemical & pharmaceutical bulletin·1992
Same author

Argyrophilic nucleolar organizer region counts in exocrine pancreatic tumors.

International journal of pancreatology : official journal of the International Association of Pancreatology·1992

Area of Science:

  • Molecular Evolution
  • Genetics
  • Bioinformatics

Background:

  • The generation-time effect proposes a link between the time between generations and the rate of molecular evolution.
  • Understanding this effect is crucial for accurately interpreting genetic divergence across species.
  • Previous studies have yielded mixed results regarding the strength and direction of the generation-time effect.

Purpose of the Study:

  • To estimate the generation-time effect separately for synonymous and nonsynonymous DNA substitutions in mammals.
  • To compare the impact of the generation-time effect across different mammalian lineages, specifically rodents and primates.
  • To evaluate the consistency of findings using multiple established methods for evolutionary analysis.

Main Methods:

  • Analysis of DNA sequences from 17 mammalian genes.

Related Experiment Videos

  • Construction of star phylogenies for rodentia, artiodactyla, and primates.
  • Application of three distinct methods (Nei and Gojobori, Li, and Ina) to estimate evolutionary parameters.

    • Main Results:

    • The generation-time effect was found to be more significant for synonymous substitutions than for nonsynonymous substitutions.
    • The proportion of accepted amino acid substitutions was estimated to be approximately twice as high in the primate lineage compared to the rodent lineage.
    • Consistent results were observed across the three analytical methods employed.

    Conclusions:

    • The generation-time effect demonstrably influences synonymous substitution rates more strongly than nonsynonymous rates in mammals.
    • The observed differences in amino acid substitution rates between primates and rodents align with predictions from the nearly neutral theory of molecular evolution.
    • This study provides robust evidence supporting the role of generation time in shaping molecular evolutionary patterns across mammalian lineages.