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Evaluation of a glucose oxidase procedure
The American Journal of Medical Technology
|October 1, 1976
Summary
This study validates a commercial glucose oxidase method for accurate glucose measurement. It shows excellent correlation with hexokinase and good agreement with automated analyzers, suitable for diverse clinical needs.
Area of Science:
- Clinical Chemistry
- Biochemical Assays
Background:
- Accurate glucose determination is crucial for diagnosing and managing diabetes.
- The glucose oxidase method offers a specific approach to glucose quantification.
Purpose of the Study:
- To evaluate a commercial adaptation of Trinder's glucose oxidase procedure for glucose determination.
- To assess its correlation with established methods and its suitability for clinical use.
Main Methods:
- Regression analysis was used to compare the commercial glucose oxidase procedure with a hexokinase reference method.
- Correlation was also assessed with various automated analyzers (AutoAnalyzer I, SMA 12/60, Beckman Glucose Analyzer) using glucose oxidase variations.
Main Results:
- Excellent correlation was observed between the commercial glucose oxidase procedure and the hexokinase method.
- Good correlation was found with automated analyzers, indicating consistent performance across different platforms.
- No interference was detected from common anticoagulants and preservatives.
Conclusions:
- The commercial glucose oxidase kit demonstrates equivalent quality to the original Trinder method.
- This validated method provides a reliable system for screening, routine, and emergency glucose testing with comparable results.