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Hydrogenase activity in nitrogen-fixing methane-oxidizing bacteria

J A Bont

    Antonie Van Leeuwenhoek
    |January 1, 1976
    PubMed
    Summary
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    Hydrogenase activity in methane-oxidizing bacteria significantly increased with nitrogen fixation. This suggests a direct link between hydrogenase and nitrogenase enzymes, crucial for nitrogen fixation processes.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Environmental Science

    Background:

    • Methane-oxidizing bacteria play a role in carbon and nitrogen cycles.
    • Nitrogen fixation is a vital process for nutrient availability in ecosystems.
    • Understanding the enzymes involved in these processes is crucial for microbial ecology.

    Purpose of the Study:

    • To investigate the hydrogenase activity in a nitrogen-fixing methane-oxidizing bacterium.
    • To explore the potential relationship between hydrogenase and nitrogenase in Methylosinus strain 41.
    • To determine the effect of hydrogen gas on acetylene reduction, an indicator of nitrogenase activity.

    Main Methods:

    • Culturing of Methylosinus strain 41 under conditions of nitrogen fixation.
    • Measurement of hydrogenase activity in bacterial cells.

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  • Assay of acetylene reduction in the presence and absence of hydrogen gas.

    • Main Results:

    • Hydrogenase activity was markedly increased when bacterial growth depended on gaseous nitrogen fixation.
    • A direct relationship between hydrogenase and nitrogenase was suggested.
    • Hydrogen gas was found to support acetylene reduction, indicating nitrogenase activity.

    Conclusions:

    • Nitrogen fixation stimulates hydrogenase activity in this bacterium.
    • Hydrogenase and nitrogenase may be functionally linked in Methylosinus strain 41.
    • Hydrogen metabolism is likely integrated with nitrogen fixation in these microorganisms.