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Comparison of methods for specific depletion of ATP in Salmonella typhimurium
1Department of Microbiology, Loma Linda University School of Medicine, California 92350.
Applied and Environmental Microbiology
|October 1, 1993
Abstract:
Three methods of ATP depletion in Salmonella typhimurium were compared. ATP concentrations were lowest after arsenate treatment. Arsenate or alpha-methylglucoside-plus-azide treatment nonspecifically lowered all nucleotide triphosphate levels. Histidine starvation in a hisF mutant was relatively specific for ATP depletion and therefore has potential in distinguishing ATP-dependent processes from processes dependent on other nucleotides.