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Lipid peroxide levels in type II hyperlipoproteinemic subjects
K Kameda-Takemura1, C N Corder, D M Lee
1Lipoprotein and Atherosclerosis Research Program, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Artery
|January 1, 1993
Summary
Baseline lipid peroxide levels are not elevated in hypercholesterolemic individuals compared to controls. This suggests that increased susceptibility to atherosclerosis in hypercholesterolemia is not due to higher baseline lipid peroxide levels.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Cardiovascular Research
Background:
- Hypercholesterolemia is a risk factor for atherosclerosis.
- Lipid peroxidation is implicated in atherogenesis.
- Baseline lipid peroxide levels in hypercholesterolemia require clarification.
Purpose of the Study:
- To determine if baseline lipid peroxide levels are higher in hypercholesterolemic subjects compared to normolipidemic controls.
- To rigorously control for in vitro oxidation during sample processing.
Main Methods:
- Two assay methods were used: thiobarbituric acid (TBA) and hemoglobin-methylene blue (HbMB).
- Plasma and serum were collected and processed under strict conditions to prevent in vitro oxidation.
- Lipid peroxide determination was performed within 2 hours of blood collection.
Main Results:
- Lipid peroxide levels were significantly higher in serum than in plasma for both hypercholesterolemic and control groups.
- No significant difference in lipid peroxide levels was observed between hypercholesterolemic and control subjects in either plasma or serum.
- No correlation was found between lipid peroxide levels and plasma cholesterol or LDL-cholesterol.
Conclusions:
- The increased tendency towards atherosclerosis in hypercholesterolemia is unlikely related to baseline plasma lipid peroxide levels.
- Lipid peroxidation during serum clot formation is higher than in plasma.
- Further research is needed to elucidate the mechanisms of atherosclerosis in hypercholesterolemia.