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[The detection of Pasteurella multocida toxin using a commercially available ELISA]
A Weber1, R Wachowitz, H Singer
1Landesuntersuchungsamt für das Gesundheitswesen Nordbayern, Nürnberg.
Berliner Und Munchener Tierarztliche Wochenschrift
|October 1, 1993
Summary
This study compared two methods for detecting toxin-producing Pasteurella multocida in pigs. ELISA identified more toxigenic strains than tissue culture, aiding in swine disease diagnosis.
Area of Science:
- Veterinary Microbiology
- Bacteriology
- Animal Pathology
Context:
- Pasteurella multocida is a significant pathogen in swine respiratory diseases.
- Accurate detection of toxigenic strains is crucial for effective disease management.
- Current diagnostic methods require comparison for optimal sensitivity and specificity.
Purpose:
- To compare the efficacy of tissue culture (EBL cells) and a commercial ELISA kit (DAKO ELISA-PMT-Kit) for identifying toxigenic Pasteurella multocida strains.
- To determine the prevalence of toxigenic P. multocida in pig nasal swabs.
Summary:
- 126 Pasteurella multocida strains from pig nasal swabs were analyzed.
- ELISA identified 13 toxigenic strains, while tissue culture identified 12.
- Toxigenic P. multocida was detected in 14 of 219 nasal swab cultures; isolation was hindered by mixed bacterial flora in 7 cases.
Impact:
- ELISA demonstrated high efficacy in detecting toxigenic P. multocida strains.
- Findings contribute to improved diagnostic strategies for swine bacterial infections.
- Highlights challenges in isolating specific bacteria from complex mixed cultures.