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Updated: Aug 18, 2026

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
Published on: October 9, 2016
Splicing of an anti-sense Alu sequence generates a coding sequence variant for the alpha-3 subunit of a neuronal
M Mihovilovic1, Y Mai, M Herbstreith
1Department of Medicine, Duke University DUMC, Durham, NC 27710.
Abstract:
In this report we demonstrate that an alpha-3 acetylcholine receptor subunit transcriptional variant originates through alternative splicing of a complementary sequence of the right arm of an Alu element. This element is located within the 5.1 Kb intron found between exons 5 and 6 of the alpha-3 acetylcholine receptor subunit gene. The transcriptional variant originates from the normal splicing process and carries an in-frame stop codon. If translated, it should encode for a peptide lacking the 4th transmembrane domain of the normal subunit.
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