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Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
Published on: February 16, 2010
Funnel-well SDS-PAGE: a rapid technique for obtaining sufficient quantities of low-abundance proteins for internal
1Laboratoire de Biologie Moléculaire et Cellulaire, Ecole Normale Supérieure de Lyon.
Biotechniques
|October 1, 1993
Abstract:
We report a modified sodium dodecyl sulfate polyacrylamide gel electrophoresis method that permits up to a 60-fold concentration factor, without significant loss of protein. This method leads to very efficient concentration of low-abundance proteins from partially purified fractions or very dilute protein solution. Furthermore, it permits in situ enzymatic digestion and consequently increases the probability of obtaining a suitable internal sequence.

