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Escherichia coli: derived murine interleukin-1 beta with N-terminus partially N alpha-acetylated
M A Lischwe1, R C Newton, J J Huang
1Du Pont Merck Pharmaceutical Company, Glenolden Laboratory, Pennsylvania 19036.
Protein Expression and Purification
|October 1, 1993
Abstract:
Two forms of murine recombinant interleukin-1 beta (mrIL-1 beta) from Escherichia coli were purified by ion exchange column chromatography; each exhibited equivalent biological activity in the murine thymocyte proliferation assay. It was determined by mass spectrometry of tryptic peptides that both retained the initiating methionine but one form was N alpha-acetylated at the N-terminus.