The rapid detection of unknown mutations in nucleic acids
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Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
Published on: March 29, 2017
1Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201.
Nature Genetics
|October 1, 1993
Abstract:
The task of identifying mutations in nucleic acid sequences is a vital component of research in mammalian genetics. With the advent of the polymerase chain reaction, several useful mutation detection techniques have evolved in recent years. The different methods have complementing strengths and a suitable procedure for virtually any experimental situation is now available.
