Absence of androgen receptors in LHRH immunoreactive neurons

Androgen has a negative feedback effect upon reproductive functions. Studies have suggested that this effect is mediated partially at the hypothalamic level. However, it is not clear whether the action is direct or indirect on LHRH neurons. In the present study, double immunocytochemistry was used to localize LHRH and androgen receptors. Out of 5892 LHRH immunoreactive neurons, no double-labelled neurons were observed. We conclude that the feedback effect of androgen on the central nervous system is mediated by indirect pathway(s).