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[A one-step chromatographic preparation of alpha-L-fucosidase from the rat epididymis]
Abstract:
An alpha-L-fucosidase has been purified from Rat epididymis homogenate in two steps: 1, ammonium sulfate precipitation (64 p. 100 saturation) 2, "DEAE-Sephadex A 50" chromatography. The purified glycosidase was obtained with good yield and with purification degree consistent with oligosaccharide on glycopeptide structural studies.