In vitro development of two-cell embryos obtained from female mice of different ages

Female mice of the IVCS strain (ddN origin), aged 90, 180, 240, 270 and 300 days, were used in this experiment. The percentages of fertilized ova per ova collected from the oviducts, on Day 1 of pregnancy, of mice aged 90 and 180 days were 100% and 91.1%, respectively. Significantly decreased rates of fertilized ova were observed in mice aged 240 to 300 days as compared to 90-day-old controls (P < 0.05). Almost all two-cell embryos collected from 90- to 270-day-old mice developed into blastocysts or expanded blastocysts, and the developmental rate from 2-cell embryo to hatched blastocyst then decreased significantly (P < 0.05). The developmental rate from 2-cell embryo to hatched blastocyst also decreased significantly in 300-day-old mice (P < 0.05). These results demonstrate differences in the viability of mouse embryos collected from mice of different ages.