Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Entamoeba histolytica: a method for isolate identification

C G Clark1, L S Diamond

  • 1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

Experimental Parasitology
|December 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Effect of Secondary Loss on Our Views of Eukaryotic Evolution.

The Biological bulletin·2017
Same author

Mindfulness-based therapy and behavioral activation: A randomized controlled trial with depressed college students.

Behaviour research and therapy·2016
Same author

Pretreatment depression severity in breast cancer patients and its relation to treatment response to behavior therapy.

Health psychology : official journal of the Division of Health Psychology, American Psychological Association·2015
Same author

Dynamics and kinetics of structure formation in molecularly tethered fluorocarbon/hydrocarbon amphiphiles.

The journal of physical chemistry. B·2012
Same author

Subtype analysis of Blastocystis isolates in Swedish patients.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2012
Same author

Manipulation of response-contingent unconditioned-stimulus intensity in human eyelid conditioning: A two-phase model analysis.

Memory & cognition·2011
Same journal

Morphological and infection responses of Psoroptes ovis var. cuniculi to Metarhizium pingshaense and peppermint-based compounds.

Experimental parasitology·2026
Same journal

Exploring polyporphyrin isolated from Carica papaya seed oil in the control of Anopheles gambiae (Diptera: Culicidae) () mosquito.

Experimental parasitology·2026
Same journal

Trypanocidal activity of nanoemulsion loaded with triterpene 3β,6β,16β-trihydroxylup-20(29)-ene from Combretum leprosum against Trypanosoma cruzi Y strain.

Experimental parasitology·2026
Same journal

Nanotechnology in the management of neglected protozoan diseases: Current advances, translational challenges, and future prospects.

Experimental parasitology·2026
Same journal

Corrigendum to "Expression profiling of GP63 isoforms with predicted nuclear localization signals in Leishmania (Viannia) braziliensis" [Experimental Parasitology 286 (2026) 109155].

Experimental parasitology·2026
Same journal

Identification of ZINC database-derived antitrypanosomal candidates targeting Trypanosoma congolense lysosomal-α-mannosidase using computational, biochemical and in vivo methods.

Experimental parasitology·2026
See all related articles

A new DNA-based method can now distinguish individual Entamoeba histolytica isolates. This technique uses genetic variations in two specific genes, aiding crucial epidemiological studies.

Area of Science:

  • Molecular Biology
  • Parasitology
  • Genetics

Background:

  • Accurate identification of Entamoeba histolytica isolates is crucial for understanding its epidemiology.
  • Existing methods may lack the discriminatory power needed for detailed strain analysis.

Purpose of the Study:

  • To develop a reliable method for differentiating individual Entamoeba histolytica isolates.
  • To leverage genetic polymorphism for strain identification.

Main Methods:

  • Developed a DNA-based typing method using the polymerase chain reaction (PCR).
  • Targeted two Entamoeba histolytica genes with internal tandemly repeated structures: the serine-rich antigen gene and the "strain specific gene".
  • Analyzed size and restriction site polymorphisms within the repetitive regions of these genes.

Related Experiment Videos

Main Results:

  • Detected extensive polymorphism in the targeted genes, indicating significant genetic variation between isolates.
  • Achieved 16 distinct DNA patterns from 18 isolates when using the two genes in combination.
  • Demonstrated the stability of these DNA patterns across various conditions, including long-term culture, axenization, cell cloning, and animal passage.

Conclusions:

  • The developed PCR-based method effectively distinguishes individual Entamoeba histolytica isolates.
  • This genetic fingerprinting technique provides a stable and reliable tool for epidemiological investigations.
  • The method's robustness ensures its utility in diverse experimental and clinical settings.