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Plasmodium falciparum: a method for the amplification of S antigens and its application to laboratory and field
1Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, England, U.K.
Experimental Parasitology
|December 1, 1993
Abstract:
Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates.