Suppression of neuronal potassium A-current by arachidonic acid

The effect of arachidonic acid on the A current (IA) has been studied in dissociated bullfrog neurons under whole-cell voltage-clamp conditions. Arachidonic acid reduced IA in a dose-dependent and reversible manner without a shift in the prepulse inactivation voltage-current relation. 1.75 microM inhibited IA by 50%, and higher concentrations caused a total suppression. In addition, arachidonic acid increased the M-current (IM), a different potassium current that does not inactivate. Neither indomethacin nor nordihydroguaiaretic acid, cyclooxygenase and lipoxygenase inhibitors respectively, prevented IA reduction. In contrast, nordihydroguaiaretic acid prevented IM enhancement. Eicosatetraynoic acid, an arachidonic acid analog that cannot be metabolized, also reduced IA. These results suggest that arachidonic acid metabolism is not required to suppress IA.