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[Primary antibody response in vitro of human blood lymphocytes]
A method for the induction of a primary in vitro antibody response toward a hapten, using human blood lymphocytes is presented. The stimulation of human lymphomonocytic cells with trinitrophenyl (TNP) conjugates of polyacrylamide beads elicits the production of IgM antibody forming cells specific for TNP, with a peak on day 7-day 9. The response is reproducible in its magnitude in the same individual. This model should facilitate clinical and fundamental studies of human antibody response.
A method for the induction of a primary in vitro antibody response toward a hapten, using human blood lymphocytes is presented. The stimulation of human lymphomonocytic cells with trinitrophenyl (TNP) conjugates of polyacrylamide beads elicits the production of IgM antibody forming cells specific for TNP, with a peak on day 7-day 9. The response is reproducible in its magnitude in the same individual. This model should facilitate clinical and fundamental studies of human antibody response.