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Cell cycle-specific association of E2F with the p130 E1A-binding protein
D Cobrinik1, P Whyte, D S Peeper
1Whitehead Institute for Biomedical Research, Cambridge Massachusetts 02142.
Genes & Development
|December 1, 1993
Summary
The study identifies p130 as the primary E2F partner in G0/G1 fibroblasts, with p107 replacing it near the G1/S border. This reveals a sequential regulation of E2F transcription factors by distinct partner proteins during the cell cycle.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- E2F transcription factors regulate cell cycle progression.
- Association with pRb and p107 proteins influences E2F activity.
- pRb and p107 are minor E2F partners in G0/G1 fibroblasts.
Purpose of the Study:
- Identify the major E2F partner protein in G0/G1 mouse fibroblasts.
- Investigate the dynamic changes in E2F-associated proteins during the cell cycle.
Main Methods:
- Affinity enrichment using an E2F-binding DNA column and adenovirus E1A oncoprotein.
- Characterization of protein properties to identify major E2F partners.
- Analysis of protein levels and complex formation at different cell cycle stages.
Main Results:
- p130 was identified as the major E2F-associated protein in G0 and early G1 fibroblasts.
- p107 replaced p130 as the major E2F partner near the G1/S border.
- Both p130-E2F and p107-E2F complexes bind cyclin-cdk kinases, including cyclin E-cdk2.
Conclusions:
- E2F transcription factors are regulated by a succession of partner proteins.
- The association of E2F with different proteins (p130, p107) occurs at defined cell cycle stages.
- This dynamic partnership regulates E2F activity and cell cycle progression.