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Related Experiment Videos

[Ion-selective microelectrodes for intracellular measurements]

T Yano1, K Nishi

  • 1Dept. of Anesthesiology, Kumamoto University Medical School, Japan.

Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
|October 1, 1993
PubMed
Summary

Miniaturized ion-selective microelectrodes enable real-time measurement of intracellular ion activities. This technique provides insights into cellular transport and physiological functions by tracking ion movement within single cells.

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Area of Science:

  • Electrochemistry
  • Cell Biology
  • Biophysics

Context:

  • Ion-selective electrodes (ISEs) are established tools for analyzing ion activities in solutions.
  • Recent advancements have led to miniaturized ISEs for intracellular measurements.
  • Double-barreled liquid ion-exchanger microelectrodes are a key focus for these applications.

Purpose:

  • To describe the fabrication principles of ion-selective microelectrodes.
  • To detail the intracellular applications of these microelectrodes.
  • To highlight the utility of double-barreled designs for simultaneous measurements.

Summary:

  • Ion-selective microelectrodes allow continuous, real-time monitoring of intracellular ion activities in single cells.
  • Measurements can be performed under various conditions, including altered extracellular fluid composition and drug exposure.

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  • Simultaneous measurement of transmembrane potential and intracellular ion activities is achievable with double-barreled ISEs.

    • Impact:

    • Facilitates the study of actual intracellular ion movement.
    • Aids in elucidating cellular mechanisms of membrane transport.
    • Contributes to understanding other vital physiological functions within cells.