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Related Experiment Videos

Intranuclear filaments containing a nuclear pore complex protein

V C Cordes1, S Reidenbach, A Köhler

  • 1Division of Cell Biology, German Cancer Research Center, Heidelberg.

The Journal of Cell Biology
|December 1, 1993
PubMed
Summary

Nuclear pore complexes (NPCs) anchor intranuclear filaments. A specific NPC protein (nup 153) forms these filaments, disassembling during mitosis and dispersing in the cytoplasm.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Nuclear pore complexes (NPCs) are crucial for nucleocytoplasmic transport.
  • Intranuclear filaments attached to NPCs vary in length across cell types.
  • The composition and dynamic behavior of these filaments are not fully understood.

Purpose of the Study:

  • To identify the protein component of intranuclear NPC-attached filaments.
  • To investigate the dynamic assembly and disassembly of these filaments during the cell cycle.
  • To explore potential functions of NPC-attached filaments.

Main Methods:

  • Monoclonal antibody-based detection of NPC proteins.
  • SDS-PAGE analysis to determine protein molecular weight.
  • Immunoprecipitation and sedimentation analysis to study protein solubility and assembly.

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Main Results:

  • A ~190 kD polypeptide, identified as nup 153, is a general constituent of intranuclear NPC-attached filaments across species.
  • This filament protein disassembles during mitosis and meiotic metaphase, forming soluble entities.
  • The soluble protein disperses throughout the cytoplasm, suggesting assembly independent of the nuclear envelope.

Conclusions:

  • Nup 153 is a key protein involved in the formation of intranuclear NPC-attached filaments.
  • NPC proteins can assemble into filaments away from the nuclear envelope, potentially serving as storage.
  • These filaments may play roles in nucleocytoplasmic transport guidance or protein storage in oocytes.