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Evaluation of MicroScan rapid panels for detection of high-level aminoglycoside resistance in enterococci
G L Woods1, B DiGiovanni, M Levison
1Department of Pathology, Medical College of Pennsylvania, Philadelphia 19129.
Abstract:
The ability of MicroScan rapid panels to detect high-level aminoglycoside-resistant enterococci was evaluated. By agar dilution, 46 of 139 isolates were susceptible to gentamicin (GNT) and streptomycin (STRP); the rest were highly resistant to one or both agents. Rapid panels detected 97.5% of STRP- and GNT-resistant isolates and had a specificity of 95.6%. Detection of resistance by conventional panels at 18 h was 64.6% for STRP and 90.2% for GNT.
Insights
MicroScan rapid panels accurately detect high-level aminoglycoside resistance in enterococci. These panels identified 97.5% of resistant isolates, outperforming conventional methods for gentamicin and streptomycin resistance.
Area of Science:
- Microbiology
- Clinical Diagnostics
- Antimicrobial Resistance
Background:
- Enterococci are significant nosocomial pathogens.
- High-level aminoglycoside resistance (HLAR) in enterococci complicates treatment.
- Accurate and rapid detection of HLAR is crucial for effective patient management.
Purpose of the Study:
- To evaluate the performance of MicroScan rapid panels for detecting high-level aminoglycoside resistance (HLAR) in enterococci.
- To compare the accuracy of rapid panels with conventional methods.
Main Methods:
- Agar dilution was used as the gold standard for determining resistance.
- 139 enterococcal isolates were tested.
- MicroScan rapid panels and conventional panels were used for detection.
Main Results:
- Agar dilution identified 46 of 139 isolates as susceptible to gentamicin (GNT) and streptomycin (STRP); the remainder showed high-level resistance.
- MicroScan rapid panels detected 97.5% of STRP- and GNT-resistant isolates with 95.6% specificity.
- Conventional panels showed lower detection rates: 64.6% for STRP and 90.2% for GNT at 18 hours.
Conclusions:
- MicroScan rapid panels offer a highly sensitive and specific method for detecting HLAR in enterococci.
- Rapid panels provide a significant improvement over conventional methods in terms of speed and accuracy.
- The use of MicroScan rapid panels can facilitate timely and appropriate therapeutic decisions for infections caused by resistant enterococci.