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Nucleotide sequence of human adenovirus type 12 DNA: comparative functional analysis
J Sprengel1, B Schmitz, D Heuss-Neitzel
1Institute of Genetics, University of Cologne, Germany.
Journal of Virology
|January 1, 1994
Summary
Researchers sequenced human adenovirus type 12 (Ad12) DNA, revealing its complete 34,125 nucleotide sequence. Comparisons with adenovirus type 2 (Ad2) DNA highlighted distinct differences and similarities in gene organization and terminal sequences.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Human adenovirus type 12 (Ad12) is a significant human pathogen.
- Understanding viral genome structure is crucial for virology research.
Purpose of the Study:
- To determine the complete nucleotide sequence of human adenovirus type 12 (Ad12) DNA.
- To compare the Ad12 genome with that of adenovirus type 2 (Ad2) and identify similarities and differences.
Main Methods:
- Ad12 DNA was isolated from first-passage human embryonic kidney cells.
- 18 PstI fragments of Ad12 DNA were cloned into the pBluescript KS vector.
- The nucleotide sequence of both DNA strands was determined using successive oligodeoxyribonucleotide primers and computer analyses.
Main Results:
- The complete Ad12 DNA sequence comprises 34,125 nucleotides with a molecular weight of approximately 22 x 10^6.
- Computer analysis identified open reading frames, leader sequences, and regulatory signals.
- Ad12 DNA exhibits an inverted terminal redundancy of 161 nucleotides, longer than Ad2's 102 nucleotides.
- Overall sequence similarity between Ad12 and Ad2 ranged from 59% to 77%, with high homology in the E2 region and adenovirus DNA polymerase sequences.
Conclusions:
- The complete Ad12 genome sequence provides a foundation for further molecular and genetic studies.
- Distinct differences in genome organization and terminal redundancy exist between Ad12 and Ad2.
- High homology in specific regions suggests conserved functions and evolutionary relationships between adenovirus types.