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[Flow cytometry in clinical laboratory medicine]
K Ohmori1, T Yoneda, S Sugiyama
1Department of Laboratory Medicine, Kyoto University, School of Medicine.
Rinsho Byori. the Japanese Journal of Clinical Pathology
|September 1, 1993
Summary
Accurate lymphocyte gating in flow cytometry is crucial for diagnosing blood disorders and leukemia. Utilizing CD13 and CD33 markers improves precision in identifying lymphocyte subsets and monitoring non-lymphoid cell contamination.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Context:
- Flow cytometry is essential for evaluating immunological functions and diagnosing leukemias.
- Accurate gating of lymphocyte subsets is critical for reliable analysis.
- Current methods may require optimization for specific patient populations like those with paroxysmal nocturnal hemoglobinuria (PNH).
Purpose:
- To recommend improved gating strategies for lymphocyte subset analysis in flow cytometry.
- To enhance the accuracy of differential diagnosis for leukemias.
- To highlight the utility of novel cell adhesion molecules in leukemia research.
Summary:
- The study proposes using CD13 and CD33, in addition to CD45 and CD14, for more precise lymphocyte gating and monitoring of non-lymphoid cell contamination, particularly in PNH patients.
- It advocates for increased use of 2-color and 3-color analyses in routine lymphocyte subset testing.
- Combining nuclear DNA content or TdT with other markers aids in analyzing minimal residual disease in leukemia.
Impact:
- Improved diagnostic accuracy for various blood disorders and leukemias.
- Enhanced monitoring of minimal residual disease in leukemia patients.
- Provides insights into the role of cell adhesion molecules in leukocyte recruitment and leukemia cell infiltration.