Voltammetric studies of redox-active centers in metalloproteins adsorbed on electrodes

Voltammetry is an electroanalytical technique in which the current flowing through an electrochemical cell is measured as a function of applied potential, typically under conditions of concentration polarization. The technique provides valuable information about redox-active species, and the current response is plotted as a voltammogram.
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...

A current produced due to the redox reactions of the analyte at the working and auxiliary electrodes is called a faradaic current. The reaction can be divided into two types. The current generated due to the reduction of the analyte is called cathodic current, and it carries a positive charge. In contrast, the current produced by analyte oxidation is known as an anodic current, and it has a negative charge. The applied potential at the working electrode determines the faradaic current flow, and...

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...

Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...

Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...

Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current.
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...