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Updated: Oct 13, 2026

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions
Published on: March 12, 2019
Characteristics of inositol polyphosphate metabolism in cultured adrenal chromaffin cells
1Department of Pharmacology, Keio University School of medicine, Tokyo, Japan.
Abstract:
1. Nicotine, high K+ and maitotoxin caused the inositol polyphosphate accumulation concomitant with 45Ca2+ uptake. 2. Angiotensin II (Ang II) and ATP induced the inositol polyphosphate accumulation without 45Ca2+ uptake. 3. Nifedipine-treatment and Ca(2+)-deprivation inhibited the high K(+)-induced inositol polyphosphate accumulation but failed to inhibit the Ang II-induced inositol polyphosphate accumulation. 4. 12-O-tetradecanoylphorbol-13-acetate inhibited the Ang II-induced inositol polyphosphate accumulation but failed to inhibit the high K(+)-induced one. 5. These results suggest that the formation of inositol polyphosphates may be regulated by two mechanisms, i.e. Ca2+ uptake-dependent mechanisms represented by high K+, and Ca2+ uptake-independent mechanisms represented by Ang II.
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