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Transformation of cholic acid by Arthrobacter simplex
E Mukherjee1, S Banerjee, S B Mahato
1Indian Institute of Chemical Biology, Calcutta.
Steroids
|October 1, 1993
Summary
Microbial fermentation of cholic acid by Arthrobacter simplex produced several known and one new metabolite. Spectroscopic methods, including 13C NMR, were used to characterize these biologically derived compounds.
Area of Science:
- Microbial chemistry
- Biocatalysis
- Natural product synthesis
Background:
- Cholic acid is a primary bile acid with potential for biotransformation.
- Microbial fermentation offers a pathway to generate novel steroid derivatives.
Purpose of the Study:
- To investigate the microbial transformation of cholic acid using Arthrobacter simplex.
- To identify and characterize the metabolites produced during this fermentation process.
Main Methods:
- Aerobic fermentation of cholic acid with Arthrobacter simplex (IICB 227).
- Isolation and purification of microbial metabolites.
- Characterization using various spectroscopic methods, including 13C NMR.
Main Results:
- Identification of eight known bile acid derivatives.
- Discovery and characterization of a novel metabolite: 2 beta-hydroxy-3,12-dioxo-23,24-dinorchola-4,6-dienoic acid.
- Complete elucidation of 13C NMR spectra for some metabolites.
Conclusions:
- Arthrobacter simplex effectively biotransforms cholic acid into various oxidized and shortened derivatives.
- The study expands the library of microbial-derived steroid compounds.
- Spectroscopic techniques are crucial for structural determination of these complex molecules.