[The isolation and identification of a Clostridium botulinum serotype A strain]

319 soil specimens were collected from different places of China for isolating Clostridium botulinum. A strain of Clostridium botulinum was isolated from a culture of soil specimens in Ruoergai of Sichuan Province, the strain was called As-3. The As-3 was identified as Clostridium botulinum serotype A according to its biological properties, biochemical serological and toxicological characteristics and DNA determination. Its DNA G + C mol is 24.9%. The toxin produced by As-3 strain can only be neutralized by type A antiserum.