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Characterization of Acinetobacter haemolyticus aac(6')-Ig gene encoding an aminoglycoside 6'-N-acetyltransferase

T Lambert1, G Gerbaud, M Galimand

  • 1Centre d'Etudes Pharmaceutiques, Chatenay-Malabry, France.

Antimicrobial Agents and Chemotherapy
|October 1, 1993
PubMed

Insights

The amikacin resistance gene acc(6')-Ig in Acinetobacter haemolyticus was characterized. This species-specific gene may be useful for identifying Acinetobacter haemolyticus strains.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Amikacin is a crucial antibiotic for treating infections caused by Gram-negative bacteria.
  • Antibiotic resistance, particularly to aminoglycosides, is a growing global health concern.
  • Acinetobacter species are opportunistic pathogens known for their intrinsic and acquired resistance mechanisms.

Purpose of the Study:

  • To characterize the amikacin resistance gene acc(6 extprime)-Ig in Acinetobacter haemolyticus BM2685.
  • To determine the genetic location and transferability of the acc(6 extprime)-Ig gene.
  • To assess the species specificity of the acc(6 extprime)-Ig gene for identifying Acinetobacter haemolyticus.

Main Methods:

  • Gene sequencing and analysis of the deduced amino acid sequence.
  • Conjugation and transformation experiments to assess gene transferability.
  • DNA hybridization (including dot blot assays) to detect the presence of the acc(6 extprime)-Ig gene in various bacterial strains.

Main Results:

  • The acc(6 extprime)-Ig gene is a 438 bp coding sequence encoding an aminoglycoside 6 extprime-N-acetyltransferase.
  • The gene was found to be chromosomally located and not transferable by conjugation or transformation.
  • The acc(6 extprime)-Ig gene was detected in all tested Acinetobacter haemolyticus strains but not in other Acinetobacter species or related bacterial families.

Conclusions:

  • The acc(6 extprime)-Ig gene is a species-specific marker for Acinetobacter haemolyticus.
  • This gene's presence can be used as a reliable method for identifying Acinetobacter haemolyticus.
  • Understanding the genetic basis of antibiotic resistance in Acinetobacter is vital for effective treatment strategies.

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