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Augmentation of murine tumor necrosis factor production by amphotericin B in vitro and in vivo
Y Tokuda1, M Tsuji, M Yamazaki
1Department of Microbiology and Immunology, School of Medicine, Teikyo University, Tokyo, Japan.
Abstract:
Murine peritoneal macrophages were preincubated with amphotericin B (AMPH) and were then stimulated with bacterial lipopolysaccharide or streptococcal preparation (OK432). These macrophages produced a large amount of tumor necrosis factor. When administered to mice, the priming activity of amphotericin B for tumor necrosis factor production in vivo was also observed.
Insights
Amphotericin B primes macrophages to produce high levels of tumor necrosis factor. This immune-boosting effect was confirmed in mice, demonstrating its potential in vivo.
Area of Science:
- Immunology
- Pharmacology
Background:
- Tumor necrosis factor (TNF) is a key cytokine in inflammatory responses.
- Amphotericin B (AMPH) is an antifungal agent with potential immunomodulatory effects.
Purpose of the Study:
- To investigate the effect of Amphotericin B on macrophage activation and TNF production.
- To assess the in vivo efficacy of Amphotericin B as an immune priming agent.
Main Methods:
- Murine peritoneal macrophages were preincubated with Amphotericin B.
- Macrophages were stimulated with bacterial lipopolysaccharide or streptococcal preparation (OK432).
- Tumor necrosis factor production was measured.
Main Results:
- Amphotericin B preincubation significantly enhanced TNF production by macrophages upon stimulation.
- In vivo studies in mice confirmed the priming activity of Amphotericin B for TNF production.
Conclusions:
- Amphotericin B acts as a potent priming agent for macrophage-mediated TNF production.
- These findings suggest a potential role for Amphotericin B beyond antifungal therapy, in modulating immune responses.