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Updated: Aug 17, 2026

An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
Published on: October 4, 2018
Studies on antigenic relatedness of classic and variant strains of infectious bursal disease viruses
1Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Abstract:
Antigenic relatedness of six classic and variant strains of serotype 1 infectious bursal disease virus (IBDV) and one serotype 2 IBDV was investigated by Western blotting and enzyme-linked immunosorbent assay (ELISA) using polyclonal, monoclonal, and monospecific antibodies to single viral proteins (VP2 and VP3). All virus strains cross-reacted similarly, and the viruses were not distinguishable from each other by ELISA or Western blot analysis performed with polyclonal or non-neutralizing monoclonal and monospecific antibodies. Non-neutralizing antibodies against the VP2 (40 kilodaltons) reacted strongly with VP2 of classic and variant strains of serotype 1 and reacted weakly with VP2 of serotype 2 OH strain. This indicated that common antigens were recognized and that these epitopes were not strictly dependent on the native structure of the virus.

