Related Concept Videos

Kidney Procurement in a Preclinical Large Animal Model05:36

Kidney Procurement in a Preclinical Large Animal Model

Here, we present a protocol that details a surgical model for kidney procurement in a preclinical swine model for subsequent machine perfusion or transplantation.
621
A Preclinical Model of Exertional Heat Stroke in Mice08:22

A Preclinical Model of Exertional Heat Stroke in Mice

The protocol describes the development of a standardized, repeatable, preclinical model of exertional heat stroke (EHS) in mice free from adverse external stimuli such as electric shock. The model provides a platform for mechanistic, preventative, and therapeutic...
4.3K
Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models07:21

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

Here we provide a detailed procedure for large-scale production of research-grade AAV vectors using adherent HEK 293 cells grown in cell stacks and affinity chromatography purification. This protocol consistently yields >1 x 1013 vector genomes/mL, providing vector quantities appropriate for large animal...
7.1K
Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis06:07

Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis

Described here is the photothrombotic stroke model, where a stroke is produced through the intact skull by inducing permanent microvascular occlusion using laser illumination after administration of a photosensitive...
7.6K
A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats10:15

A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats

The purpose of this study is to establish and validate an animal model for research in the recovery and sequela stages of brain ischemia by testing brain infarction and sensorimotor function after middle cerebral artery occlusion/reperfusion (MCAO/R) after 1-90 days in...
8.8K
Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery08:14

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

Various murine models of middle cerebral artery occlusion (MCAO) are widely used in experimental brain research. Here, we demonstrate the model of transcranial permanent distal MCAO which produces consistent cortical infarction of a size corresponding to damage imposed by the majority of human ischemic...
38.4K