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A model for cleavage of O-glycosidic bonds in glycoproteins
F M Ibatullin1, A M Golubev, L M Firsov
1Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Gatchina, Russia.
Glycoconjugate Journal
|June 1, 1993
Abstract:
The present work investigated the possibility of cleavage of alpha-linkages between mannose or galactose and serine/threonine residues by alpha-mannosidase and alpha-galactosidase. The study was carried out initially with model synthetic compounds imitating the O-glycosidic bond in glycoproteins, and further with glucoamylase. It was shown that alpha-mannosidase and alpha-galactosidase can hydrolyse these linkages after proteolytic digestion of glucoamylase.