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Developmental changes in neutral glycosphingolipids of mouse placenta
J Svejcar1, S Ehrlich-Rogozinski, D Riedel
1Institute for Human Genetics, Johann Wolfgang Goethe University, Frankfurt am Main, Germany.
Glycoconjugate Journal
|June 1, 1993
Summary
Mouse placental glycosphingolipid analysis reveals the appearance of the Forssman antigen on day 12 of gestation. This change coincides with placental maturation, suggesting its role in embryonic development.
Area of Science:
- Developmental Biology
- Glycobiology
- Mammalian Reproduction
Background:
- The mammalian placenta is crucial for studying embryonic development.
- Mouse models offer precise gestational staging for placental research.
- Understanding placental composition changes is key to developmental insights.
Purpose of the Study:
- To investigate changes in neutral glycosphingolipids during mouse placental development.
- To identify specific glycosphingolipids present from day 10 to day 18 of gestation.
- To explore the potential role of identified glycosphingolipids in placental maturation.
Main Methods:
- Isolation and separation of neutral glycosphingolipids from C57BL/6 mouse placentas using high-performance thin-layer chromatography (HPTLC).
- Densitometric analysis of orcinol-stained glycosphingolipids.
- Identification of the Forssman antigen using Helix pomatia agglutinin (HPA) binding and monoclonal antibody assays.
Main Results:
- Glycosphingolipids including mono-, tetra-, tri-, and dihexosylceramide were detected on day 10.
- Four unidentified spots were observed on day 10, disappearing by day 12.
- A Forssman antigen-like glycosphingolipid appeared on day 12, with no further changes until day 18.
Conclusions:
- The Forssman antigen emerges in the mouse placenta at day 12 of gestation.
- This emergence coincides with the final maturation and growth cessation of the placenta.
- The Forssman antigen may play a significant role in mammalian embryonic development and placental function.