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Related Experiment Videos

Is TPO detectable in the circulation?

L D Premawardhana1, Y Kiso, D I Phillips

  • 1Endocrine Immunology Unit, University of Wales College of Medicine, Heath Park, Cardiff, U.K.

Thyroid : Official Journal of the American Thyroid Association
|January 1, 1993
PubMed
Summary
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Thyroid peroxidase (TPO) is generally undetectable in circulation for normal individuals and Graves' disease patients. Assay interference from TPO autoantibodies can cause false positives, and TSH does not trigger TPO release.

Area of Science:

  • Endocrinology
  • Immunology
  • Clinical Chemistry

Background:

  • Recent reports suggested circulating thyroid peroxidase (TPO) in normal subjects and Graves' disease patients.
  • Thyroid peroxidase is a key enzyme in thyroid hormone synthesis.

Purpose of the Study:

  • To confirm and extend previous findings on circulating TPO.
  • To investigate TPO detectability in normal and Graves' disease sera.
  • To assess the impact of TSH stimulation on TPO release.

Main Methods:

  • Radioimmunoassay (RIA) for TPO measurement with 1 ng/mL sensitivity.
  • Analysis of sera from 20 normal subjects and 21 Graves' disease patients.
  • Measurement of TPO in normal subjects before and after TRH stimulation.

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Main Results:

  • TPO was undetectable in 46 out of 47 sera analyzed.
  • One false positive result in a normal subject was attributed to TPO autoantibody interference.
  • No significant TPO release was observed after TRH-induced TSH increase.

Conclusions:

  • Thyroid peroxidase is not detectable in circulation of normal subjects or TPO autoantibody-negative Graves' disease patients.
  • Endogenous TPO autoantibodies can interfere with TPO radioimmunoassays, causing false positives.
  • Acute TSH increase does not lead to TPO release into circulation.