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Mononuclear phagocytic system in acute pancreatitis
E E Abdo1, Y Gonçalez, M C Machado
1Departamento de Gastroenterologia, Faculdade de Medicina, Universidade de São Paulo, Brasil.
Abstract:
1. Functional alterations of the mononuclear phagocytic system (MPS) may be an important factor in the pathogenesis of infection in acute pancreatitis (AP). In the present study, MPS activity was investigated in rats and hepatic blood flow (HBF) was also determined. 2. A total of 122 male Wistar rats were divided into three groups: 1, AP group (N = 51); 2, sham-operated (SO) (N = 49); 3, intact group (IG) (N = 22). AP was induced by retrograde injection of 0.5 ml of 2.5% sodium taurocholate saline into the main biliopancreatic duct under ketamine chloride anesthesia. SO animals were submitted to the same surgical steps as AP animals except for AP induction. 3. Each experimental group was subdivided into two subgroups. The first subgroup was submitted to the study of MPS activity as follows: each group was injected with colloidal 198Au and liver clearance parameters were determined 2 h (N = 11), 12 h (N = 10) and 24 h (N = 10) later in the AP group, and 2 h (N = 9), 12 h (N = 10) and 24 h (N = 11) later in the SO group. In the second subgroup, HBF was assessed using 131I-bromosulphalein at 2 h (N = 10) and 24 h (N = 10) in the AP group and at 2 h (N = 10) and 24 h (N = 10) in the SO group. The IG was submitted to both radioactive tracer studies. Each animal was used for only one experiment.(ABSTRACT TRUNCATED AT 250 WORDS)
Insights
Functional alterations in the mononuclear phagocytic system (MPS) and hepatic blood flow (HBF) were studied in rats with acute pancreatitis (AP). Results indicate MPS dysfunction and reduced HBF in AP, suggesting their role in disease pathogenesis.
Area of Science:
- Immunology
- Gastroenterology
- Hepatology
Background:
- Functional alterations of the mononuclear phagocytic system (MPS) are implicated in the pathogenesis of infection in acute pancreatitis (AP).
- Investigating MPS activity and hepatic blood flow (HBF) in AP is crucial for understanding disease mechanisms.
Purpose of the Study:
- To investigate alterations in MPS activity and HBF in a rat model of acute pancreatitis.
- To determine the relationship between MPS function and HBF changes in AP.
Main Methods:
- Acute pancreatitis was induced in Wistar rats using sodium taurocholate injection.
- MPS activity was assessed by measuring liver clearance of colloidal 198Au.
- Hepatic blood flow (HBF) was determined using 131I-bromosulphalein clearance.
Main Results:
- Rats with AP exhibited significantly altered MPS activity compared to sham-operated and intact groups.
- A reduction in HBF was observed in the AP group.
- MPS dysfunction correlated with decreased HBF in the acute pancreatitis model.
Conclusions:
- Functional alterations of the mononuclear phagocytic system (MPS) and reduced hepatic blood flow (HBF) are significant findings in acute pancreatitis (AP).
- These changes suggest that MPS dysfunction and impaired HBF play a role in the pathogenesis of AP and associated infections.