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Colon Ascendens Stent Peritonitis (CASP) - a Standardized Model for Polymicrobial Abdominal Sepsis
Published on: December 18, 2010
Participation of macrophages in chloramphenicol-potentiated carrageenin-induced peritonitis in rats
J R Moraes1, F R Moraes, G H Bechara
1Departamento de Patologia Veterinária, Faculdade de Ciências Agárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brasil.
Abstract:
1. We investigated the possible potentiating effect of chloramphenicol succinate (30 mg/kg, every 12 h for 4 days, ip) on the response of polymorphonuclear neutrophils to carrageenin (150 micrograms, ip) or dextran (100 micrograms, ip) in the peritoneal cavity of male Wistar rats (180-230 g; N = 12 in each group). 2. Chloramphenicol potentiated the cell migration induced by carrageenin (35%) but not that induced by dextran. Previous macrophage depletion in the peritoneal cavity by washing with sterile saline abolished the cell response, whereas a previous thioglycollate-induced increase in macrophage numbers enhanced the potentiating effect (60%). 3. These results suggest that the potentiating effect on polymorphonuclear neutrophil migration induced by chloramphenicol may be related to chemotactic factors released by macrophages.
Insights
Chloramphenicol succinate enhances polymorphonuclear neutrophil migration in rats, particularly when macrophages are present. This suggests a role for macrophage-derived factors in the antibiotic
Area of Science:
- Immunology
- Pharmacology
Background:
- Polymorphonuclear neutrophils (PMNs) are key immune cells involved in inflammation.
- Understanding factors that modulate PMN migration is crucial for developing anti-inflammatory strategies.
Purpose of the Study:
- To investigate the potential of chloramphenicol succinate to potentiate PMN migration.
- To explore the role of macrophages in chloramphenicol's effect on PMN migration.
Main Methods:
- Male Wistar rats were administered chloramphenicol succinate (30 mg/kg, every 12 h for 4 days, ip).
- Inflammation was induced using carrageenan or dextran (ip).
- Peritoneal lavage was performed to assess PMN and macrophage counts; macrophage depletion and enhancement models were used.
Main Results:
- Chloramphenicol succinate significantly potentiated carrageenan-induced PMN migration by 35%.
- This potentiation was not observed with dextran-induced inflammation.
- Macrophage depletion abolished the potentiating effect, while increased macrophage numbers enhanced it by 60%.
Conclusions:
- Chloramphenicol succinate potentiates PMN migration in response to certain inflammatory stimuli.
- The effect appears to be mediated by macrophages, suggesting chloramphenicol may influence the release of macrophage-derived chemotactic factors.
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