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Mouse hepatitis virus 3 and interferon gamma binding to extracted macrophage proteins correlate with virus growth in
M A Lucchiari1, J P Martin, M Modolell
1Instituto Butantan, Laboratório de Immunologia Viral, São Paulo, Brasil.
Abstract:
1. After immunization, adult A/J mice are resistant and BALB/c mice are susceptible to MHV3 infection. After IFN gamma activation, only macrophages originating from A/J mice were able to partially restrict MHV3 growth. 2. When the binding of MHV3 and interferon (IFN) gamma to solubilized cytoplasmic and membrane macrophage proteins of mice was determined by ELISA, there was more binding of MHV3 to proteins extracted from BALB/c macrophages than to proteins extracted from A/J macrophages. When the proteins were obtained from IFN gamma-activated macrophages, decreased MHV3 binding was observed only in proteins originating from A/J macrophages. 3. ELISA showed a comparable binding of IFN gamma to A/J or BALB/c macrophage proteins. When the proteins were obtained from IFN gamma-activated macrophages, only IFN gamma-binding to A/J macrophage proteins was increased. 4. The results indicate a different expression and IFN gamma modulation of MHV3 receptors in macrophages from A/J and BALB/c mice, which directly correlated with their acquired resistance or susceptibility to MHV3 infection.
Insights
Murine hepatitis virus 3 (MHV3) susceptibility in mice depends on macrophage receptor expression. Interferon-gamma (IFN-γ) differentially modulates these receptors, influencing resistance or susceptibility to MHV3 infection.
Area of Science:
- Immunology
- Virology
- Cell Biology
Background:
- Murine hepatitis virus 3 (MHV3) infection outcomes vary between mouse strains, with A/J mice exhibiting resistance and BALB/c mice showing susceptibility.
- Macrophages play a critical role in the innate immune response to viral infections.
Purpose of the Study:
- To investigate the differential expression and interferon-gamma (IFN-γ) modulation of MHV3 receptors on macrophages from resistant (A/J) and susceptible (BALB/c) mouse strains.
- To correlate receptor expression patterns with the observed resistance or susceptibility to MHV3 infection.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to quantify the binding of MHV3 and IFN-γ to macrophage proteins.
- Macrophage proteins were extracted from both naive and IFN-γ-activated macrophages of A/J and BALB/c mice.
Main Results:
- BALB/c macrophages exhibited higher MHV3 binding than A/J macrophages.
- IFN-γ activation led to decreased MHV3 binding in A/J macrophages but not BALB/c macrophages.
- IFN-γ binding to macrophage proteins was comparable between strains, but IFN-γ activation specifically increased IFN-γ binding to A/J macrophage proteins.
Conclusions:
- Macrophage MHV3 receptor expression differs significantly between A/J and BALB/c mice.
- IFN-γ differentially modulates MHV3 receptor expression in a strain-dependent manner.
- These differences in receptor expression and IFN-γ modulation directly correlate with the varying susceptibility of A/J and BALB/c mice to MHV3 infection.