Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chronic peripheral lymphatic cannulation in the dog

C H Sloop1, M Lefevre, C K Castle

  • 1Division of Lipoprotein Metabolism and Pathophysiology, Louisiana State University Medical Center, Baton Rouge.

Lymphology
|September 1, 1993
PubMed
Summary

A novel lymphatic-lymphatic (L-L) shunt allows prolonged collection of peripheral lymph in unanesthetized dogs. This method yields significantly higher lymph flow rates, crucial for various physiological studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Third-trimester prenatal brain imaging for early diagnosis of glutaric aciduria type 1 in monochorionic diamniotic twins.

Hong Kong medical journal = Xianggang yi xue za zhi·2026
Same author

INVESTIGATING FACTORS INFLUENCING THE AMOUNT OF INJECTION PAIN IN THE ORAL CAVITY: A SYSTEMATIC REVIEW.

Georgian medical news·2025
Same author

Longitudinal fetal growth parameters in women at high risk for pre-eclampsia.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2025
Same author

Prediction of pre-eclampsia using maternal hemodynamic parameters at 12 + 0 to 15 + 6 weeks.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2025
Same author

Consolidated and updated ultrasonographic fetal biometry and estimated fetal weight references for the Hong Kong Chinese population.

Hong Kong medical journal = Xianggang yi xue za zhi·2024
Same author

The clinical meaning and significance of OVEMP morphology.

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·2024

Area of Science:

  • Physiology
  • Surgical Innovation
  • Lymphatic System Research

Background:

  • Peripheral lymph is commonly used to represent non-visceral interstitial fluid.
  • Acute collection methods limit long-term or specific experimental needs.
  • Previous studies often relied on anesthetized subjects, potentially altering physiological parameters.

Purpose of the Study:

  • To develop and validate a method for collecting peripheral lymph over extended periods (3-5 days) in unanesthetized dogs.
  • To assess the feasibility and efficacy of a prenodal lymphatic-lymphatic (L-L) shunt for continuous lymph collection.
  • To compare lymph flow rates and protein concentrations in unanesthetized versus anesthetized states.

Main Methods:

  • Development of a prenodal lymphatic-lymphatic (L-L) shunt using medical-grade silicone rubber tubing with a slip coupling.

Related Experiment Videos

  • Surgical implantation of the shunt in dogs, allowing for disconnection and reconnection for lymph collection.
  • Collection of peripheral lymph over 3-5 days in unanesthetized dogs, monitoring flow rates and protein concentrations.

    • Main Results:

    • The L-L shunt enabled successful collection of peripheral lymph for 3-5 days in unanesthetized dogs.
    • Average peripheral lymph flow rate (4.9 ml/hr/leg) was nearly double that observed in previously studied anesthetized dogs.
    • Lymph/plasma total protein concentration ratio (0.16) remained consistent with prior findings in anesthetized dogs.
    • Lymph protein concentration exhibited diurnal variation, decreasing during collection and concentrating overnight.
    • Significant volumes of peripheral lymph (over 200 ml/dog) were collected.

    Conclusions:

    • The developed L-L shunt is an effective tool for collecting prenodal lymph in unanesthetized dogs, overcoming limitations of acute sampling.
    • This method supports research requiring unanesthetized subjects, such as feeding studies, or when large lymph volumes are needed.
    • The shunt facilitates longitudinal studies and repeated protocols on the same animal, enhancing experimental efficiency and data richness.