Related Experiment Videos
Efficacy of a disinfectant wipe method for the removal of adenovirus 8 from tonometer tips
A B Threlkeld1, J W Froggatt, O D Schein
1Wilmer Ophthamological Institute, Johns Hopkins University and Hospital, Baltimore, MD.
Purpose:
To determine the efficacy of a disinfectant wipe method in eliminating adenovirus 8 from both Goldmann tonometer and pneumotonometer tips, and to determine the efficacy of 5-minute disinfectant soaks in removing the virus from Goldmann tonometer tips.
Method:
Quantification of adenovirus 8 in the ocular secretions of six infected patients was performed. Clinically relevant inocula of adenovirus 8 were then prepared in a serum suspension which was used to contaminate Goldmann tonometer and pneumotonometer tips. Each contaminated tip was subjected to either a dry wipe or a wipe with one of the following: water, isopropyl alcohol, hydrogen peroxide, and iodophor. The tips were than quantitatively assayed for residual adenovirus 8. Disinfectant soaks were similarly assessed using contaminated Goldmann tonometer tips soaked in water, hydrogen peroxide, iodophor, and sodium hypochlorite.
Results:
Adenovirus 8 was recovered from both types of tonometer tips after dry wipes, but no viable virus was recovered from tips wiped with water only or with any of the disinfectants tested. Adenovirus 8 also was recovered from Goldmann tonometer tips that were either rinsed or soaked in water; no virus was recovered from tips soaked for 5 minutes in any of the disinfectants tested.
Conclusion:
A disinfectant wipe method using isopropyl alcohol, hydrogen peroxide, or iodophor is effective in removing adenovirus 8 from both Goldmann tonometer and pneumotonometer tips. Five-minute disinfectant soaks also are effective for Goldmann tonometer tips.
Insights
Disinfectant wipes effectively eliminate adenovirus 8 from tonometer tips. A 5-minute soak in disinfectants also removes adenovirus 8 from Goldmann tonometer tips, ensuring instrument safety.
Area of Science:
- Ophthalmology
- Infectious Disease Control
- Microbiology
Background:
- Adenovirus 8 is a significant cause of epidemic keratoconjunctivitis.
- Ophthalmic instruments like tonometer tips can harbor and transmit viruses.
- Effective disinfection protocols are crucial for preventing cross-contamination in eye care settings.
Purpose of the Study:
- To evaluate the efficacy of disinfectant wipes in eliminating adenovirus 8 from Goldmann and pneumotonometer tips.
- To assess the effectiveness of 5-minute disinfectant soaks in removing adenovirus 8 from Goldmann tonometer tips.
Main Methods:
- Adenovirus 8 was quantified in patient samples and used to contaminate tonometer tips.
- Contaminated tips underwent dry wiping, wiping with water, or wiping with isopropyl alcohol, hydrogen peroxide, or iodophor.
- Goldmann tonometer tips were also subjected to 5-minute soaks in water, hydrogen peroxide, iodophor, or sodium hypochlorite.
Main Results:
- No viable adenovirus 8 was recovered from tonometer tips wiped with water or any tested disinfectants.
- Adenovirus 8 was recovered from tips after dry wiping or rinsing/soaking in water.
- Five-minute disinfectant soaks effectively removed adenovirus 8 from Goldmann tonometer tips.
Conclusions:
- Disinfectant wipes with isopropyl alcohol, hydrogen peroxide, or iodophor are effective for adenovirus 8 removal from tonometer tips.
- A 5-minute disinfectant soak is also an effective method for cleaning Goldmann tonometer tips.
- These findings support the use of specific disinfectants to ensure ophthalmic instrument safety.