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Colocalization of NGF receptor with VIP in rat suprachiasmatic neurones
1Department of Neuroendocrinology, Hungarian Academy of Sciences, Budapest.
Abstract:
Low-affinity nerve growth factor receptor (p75NGFR) and vasoactive intestinal polypeptide (VIP) immunoreactive neuronal structures and their interrelationship were investigated at light and electron microscopic levels in rat suprachiasmatic nucleus (SCN). p75NGFR immunoreactive neuronal perikarya were detected in the ventrolateral part, while the dorsolateral region contained mainly receptor positive fibres. Double-label immunocytochemistry showed that nearly all p75NGFR positive neurones in the ventrolateral region of the SCN also contained VIP. The axons of the receptor positive neurones terminated on unidentified neurones. In single-label experiments, the axons of the VIP containing neurones formed axodendritic synapses on cells containing the same peptide. The findings provide further insight on the chemical structural organization of the SCN.