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Sequence analysis of two tandemly linked Em genes from wheat
1Department of Genetics, Leeds University, UK.
Plant Molecular Biology
|December 1, 1993
Summary
Researchers analyzed DNA sequences for two wheat Em genes at the Xem-1AL locus. These genes show high homology in protein-coding areas and possess ABA-responsive elements in their 5-flanking regions.
Area of Science:
- Plant molecular biology
- Genomics
- Wheat genetics
Background:
- The wheat Em gene family plays a role in plant development.
- Understanding gene regulation is crucial for crop improvement.
Purpose of the Study:
- To sequence and analyze two linked wheat Em genes at the Xem-1AL locus.
- To investigate sequence homology and regulatory elements within these genes.
Main Methods:
- DNA sequencing
- Comparative sequence analysis
- Bioinformatic identification of cis-acting elements
Main Results:
- The two linked wheat Em genes at the Xem-1AL locus were sequenced.
- Substantial homology was observed in the protein-coding regions of these Em genes.
- Characteristic ABA-responsive cis-acting elements were identified in the 5'-flanking regions.
Conclusions:
- The sequenced wheat Em genes share conserved protein-coding regions.
- The presence of ABA-responsive elements suggests a role in stress or developmental signaling pathways.