Studies on human platelets stored at 20-22 degrees C without agitation

Area of Science:

• Transfusion Medicine
• Hematology
• Biotechnology

Background:

• Platelet storage conditions significantly impact post-transfusion viability and function.
• Understanding early changes in stored platelets is crucial for optimizing transfusion outcomes.

Purpose of the Study:

• To evaluate the functional and biochemical changes in human platelets stored at ambient temperature without agitation for up to 96 hours.
• To identify key parameters that best reflect early storage-induced alterations and predict in vivo recovery.

Main Methods:

• Human platelets in ACD plasma were stored in sterile plastic bags at ambient temperature (20-22°C) without agitation for 24-96 hours.
• Assessed parameters included platelet count, aggregation responses (ADP, thrombin, collagen, epinephrine), platelet factor 3 activity, osmotic shock response, volumetric constants, UV-absorbent material, radiolabeled serotonin and adenosine uptake/release, population pattern, and glycogen synthesis.

Main Results:

• A progressive decline in platelet count and aggregation capacity was observed.
• Significant losses in platelet factor 3 activity, osmotic shock response, and uptake/release of radiolabeled compounds occurred.
• Platelet aggregation, release, osmotic shock test, and population pattern changes correlated with the 25-42% in vivo recirculation of 24-hour stored platelets.

Conclusions:

• Platelets stored at ambient temperature without agitation undergo significant functional and biochemical degradation within 96 hours.
• Platelet aggregation, release, osmotic shock, and population pattern are sensitive indicators of early storage lesions.
• Despite functional losses, stored platelets may exhibit paradoxical in vivo recuperation, as indicated by survival and hemostatic effects.