Related Experiment Videos
[A contribution to formation of artifacts in the autoradiography (author's transl)]
Acta Histochemica
|January 1, 1976
Abstract:
KRAUS and GIESE - as proposed by O'CALLAGHAN, STEVENS and WOOD-- could prevent latent image fading throughout exposure with 89Sr/90 Y by addition of 10 mg KBr and 50 g Glucose per liter of water for flotation of stripping films. This method is not useful for the autoradiography of biological material labelled with tritium. Autoradiograms of rat brain slices were investigated after application 3H-leucine, 3H-methionine, 3H-asparagic-acid and 3H-histidine. By adding the above mentioned salts there was an increase of artifacts and a decrease in reproducibility.